|
| Status |
Public on Jan 09, 2013 |
| Title |
Cort+RU 4HR #2 |
| Sample type |
RNA |
| |
|
| Source name |
Cortical astrocyte culture
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57B/6
tissue: Brain
developmental stage: P0-P2
treatment: 4 hour 100nM corticosterone + 500nM RU486
|
| Treatment protocol |
100nM corticosterone with or without 500nM RU486 in media for given time period (returned to incubator)
|
| Growth protocol |
Primary cultures derived from P0-P2 mouse pups (cortex or hippocampus), plated 250,000-500,000/well in 6-well dishes, grown 9-11 days until ~confluent (grown in 37C incubator with DMEM+10%FBS media; 100% media changed 24 hours post-plating, 50% media replaced every 2-3 days afterward)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion's TotalPrep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol (MouseWG-6 v2.0 Expression BeadChip)
|
| Scan protocol |
Standard Illumina scanning protocol (BeadArray reader)
|
| Description |
4 hour steroid+antagonist replicate-2
|
| Data processing |
Data normalised using quantile normalization with Illumina GenomeStudio software; for each time point, the Cort/Cort+RU data was normlized to the corresponding vehicle control data (e.g. Matrix normalized data is representative for 2 hour data; normalized to 2 hour vehicle data)
|
| |
|
| Submission date |
Jul 11, 2012 |
| Last update date |
Jan 09, 2013 |
| Contact name |
Bradley S Carter |
| E-mail(s) |
bscarter@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Molecular and Behavioral Neuroscience Institute
|
| Lab |
Thompson
|
| Street address |
109 Zina PItcher Place
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE39270 |
Corticosterone regulation of mRNAs and and corresponding RU486 antagonism in astrocytes in vitro |
|
