Klc1 kinesin light chain 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Klc1provided by MGI
Official Full Name: kinesin light chain 1provided by MGI
Primary source: MGI:MGI:107978
See related: Ensembl:ENSMUSG00000021288 AllianceGenome:MGI:107978
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Kns2
Summary: Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Expression: Broad expression in frontal lobe adult (RPKM 53.0), CNS E18 (RPKM 52.5) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 F1; 12 61.13 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (111724869..111774278)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (111758435..111807844)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Kinesin light chain 1 stabilizes insulin receptor substrate 1 to regulate the IGF-1-AKT signaling pathway during myoblast differentiation.
Title: Kinesin light chain 1 stabilizes insulin receptor substrate 1 to regulate the IGF-1-AKT signaling pathway during myoblast differentiation.
The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.
Title: The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.
Drp1 overexpression induces desmin disassembling and drives kinesin-1 activation promoting mitochondrial trafficking in skeletal muscle.
Title: Drp1 overexpression induces desmin disassembling and drives kinesin-1 activation promoting mitochondrial trafficking in skeletal muscle.
Kinesin-1-mediated axonal transport of CB1 receptors is required for cannabinoid-dependent axonal growth and guidance.
Title: Kinesin-1-mediated axonal transport of CB1 receptors is required for cannabinoid-dependent axonal growth and guidance.
DOC2B collaborates with KLC1 to regulate insulin-stimulated GLUT4 accumulation at the plasma membrane and regulates insulin sensitivity
Title: DOC2B promotes insulin sensitivity in mice via a novel KLC1-dependent mechanism in skeletal muscle.
kinesin-1 recognizes a novel class of adaptor motifs 'Y-acidic' (tyrosine flanked by acidic residues), in a KLC-isoform-specific manner.
Title: Structural basis for isoform-specific kinesin-1 recognition of Y-acidic cargo adaptors.
KLC1 deletion leads to a reduction of Mn2+ transport from CA3 to the septal nuclei.
Title: Hippocampal to basal forebrain transport of Mn(2+) is impaired by deletion of KLC1, a subunit of the conventional kinesin microtubule-based motor.
Phosphorylation of KLC1 at Thr466 increased in aged brains, and JIP1 binding to kinesin-1 decreased, suggesting that APP transport is impaired by aging. Authors conclude that phosphorylation of KLC1 at Thr466 regulates the velocity of transport of APP by kinesin-1 by modulating its interaction with JIP1b.
Title: Phosphorylation of KLC1 modifies interaction with JIP1 and abolishes the enhanced fast velocity of APP transport by kinesin-1.
In old mice, lack of KLC1 results in retinal pigment epithelium pathogenesis that was strikingly comparable to aspects of age-related macular degeneration.
Title: Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis.
Alcalpha is efficiently processed in part to minimize the inappropriate peripheral retention of kinesin-1
Title: Constitutive cleavage of the single-pass transmembrane protein alcadeinα prevents aberrant peripheral retention of Kinesin-1.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Endonuclease-mediated (1)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI182522 (e-PCR)
- UniSTS:160263

NoName (e-PCR)
- UniSTS:236963

AI874768 (e-PCR)
- UniSTS:165849

RH129416 (e-PCR)
- UniSTS:212723

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables kinesin binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables tubulin binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within axo-dendritic transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in intracellular protein transport	ISO Inferred from Sequence Orthology more info
involved_in microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule-based movement	TAS Traceable Author Statement more info	PubMed
involved_in protein localization to synapse	ISO Inferred from Sequence Orthology more info
involved_in stress granule disassembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in ciliary rootlet	IDA Inferred from Direct Assay more info	PubMed
located_in ciliary rootlet	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in growth cone	ISO Inferred from Sequence Orthology more info
part_of kinesin complex	IDA Inferred from Direct Assay more info	PubMed
part_of kinesin complex	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane-bounded organelle	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: kinesin light chain 1

Names: KLC 1; kinesin 2 60/70kDa

NP_001020529.2

EC 2.7.1.21

NP_001020530.2

EC 2.7.1.21

NP_001020531.2

EC 2.7.1.21

NP_001020532.2

EC 2.7.1.21

NP_001020533.2

EC 2.7.1.21

NP_001020534.2

EC 2.7.1.21

NP_001075428.1

EC 2.7.1.21

NP_001348534.1

EC 2.7.1.21

NP_001348538.1

EC 2.7.1.21

NP_001348539.1

EC 2.7.1.21

NP_001348540.1

EC 2.7.1.21

NP_001348541.1

EC 2.7.1.21

NP_001348542.1

EC 2.7.1.21

NP_001348543.1

EC 2.7.1.21

NP_001409078.1

EC 2.7.1.21

NP_032476.2

EC 2.7.1.21

XP_030102425.1

EC 2.7.1.21

XP_030102426.1

EC 2.7.1.21

XP_036013090.1

EC 2.7.1.21

XP_036013091.1

EC 2.7.1.21

XP_036013092.1

EC 2.7.1.21

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001025358.2 → NP_001020529.2 kinesin light chain 1 isoform 1B

Status: REVIEWED

Description

Transcript Variant: This variant (b) uses an alternate splice site and alternate exon in the 3' coding region and 3' UTR, compared to variant d. The resulting protein (isoform 1B) is shorter and has a distinct C-terminus, compared to isoform 1D.

Source sequence(s)

AA059822, BE946775, BG094009, BK000669, BU922789, BY014721, CF615940, CK620595, CV883867

UniProtKB/TrEMBL

Q7M702

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13374
Location:296 → 337

TPR_10; Tetratricopeptide repeat

pfam13424
Location:251 → 329

TPR_12; Tetratricopeptide repeat
NM_001025359.2 → NP_001020530.2 kinesin light chain 1 isoform 1C

See identical proteins and their annotated locations for NP_001020530.2

Status: REVIEWED

Description

Transcript Variant: This variant (c) uses an alternate splice site and alternate exon in the 3' coding region and 3' UTR, compared to variant d. The resulting protein (isoform 1C) is shorter and has a distinct C-terminus, compared to isoform 1D.

Source sequence(s)

BB457558, BE946775, BG094009, BK000670, BY014721, CF615940, CK032061, W10467

Consensus CDS

CCDS70423.1

UniProtKB/TrEMBL

E9Q7C9, Q7M701

Related

ENSMUSP00000113237.3, ENSMUST00000120544.9

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13374
Location:296 → 337

TPR_10; Tetratricopeptide repeat

pfam13424
Location:251 → 329

TPR_12; Tetratricopeptide repeat
NM_001025360.2 → NP_001020531.2 kinesin light chain 1 isoform 1D

See identical proteins and their annotated locations for NP_001020531.2

Status: REVIEWED

Description

Transcript Variant: This variant (d) represents the longest transcript and encodes the longest isoform (1D).

Source sequence(s)

AC152065, AK031309, AU035615

Consensus CDS

CCDS36565.1

UniProtKB/TrEMBL

Q7M700, Q8CD76

Related

ENSMUSP00000082004.6, ENSMUST00000084941.12

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13374
Location:296 → 337

TPR_10; Tetratricopeptide repeat

pfam13424
Location:251 → 329

TPR_12; Tetratricopeptide repeat
NM_001025361.2 → NP_001020532.2 kinesin light chain 1 isoform 1E

Status: REVIEWED

Description

Transcript Variant: This variant (e) uses an alternate in-frame splice site in the 3' coding region, compared to variant d. The resulting protein (isoform 1E) is shorter than isoform 1D.

Source sequence(s)

AV319299, BB835417, BG094009, BK000672, BU922789, BY014721, CF615940

UniProtKB/TrEMBL

Q7M6Z9

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13374
Location:296 → 337

TPR_10; Tetratricopeptide repeat

pfam13424
Location:251 → 329

TPR_12; Tetratricopeptide repeat
NM_001025362.2 → NP_001020533.2 kinesin light chain 1 isoform 1G

See identical proteins and their annotated locations for NP_001020533.2

Status: REVIEWED

Description

Transcript Variant: This variant (g) uses an alternate splice site, lacks an alternate exon in the 3' coding region and 3' UTR, and has an alternate exon in the 3' UTR, compared to variant d. The resulting protein (isoform 1G) is shorter and has a distinct C-terminus, compared to isoform 1D.

Source sequence(s)

BG094009, BK000674, BU922789, BY014721, CF615940

UniProtKB/TrEMBL

Q7M6Z7

Conserved Domains (3) summary

TIGR02168
Location:35 → 149

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat
NM_001025363.2 → NP_001020534.2 kinesin light chain 1 isoform 1F

Status: REVIEWED

Description

Transcript Variant: This variant (f) uses an alternate in-frame splice site and alternate exon in the 3' coding region and 3' UTR, compared to variant d. The resulting protein (isoform 1F) is shorter and has a distinct C-terminus, compared to isoform 1D.

Source sequence(s)

AA059822, BE946775, BG094009, BK000673, BY014721, CF615940, CK032061, CK620595, CV883867

UniProtKB/TrEMBL

Q7M6Z8

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13374
Location:296 → 337

TPR_10; Tetratricopeptide repeat

pfam13424
Location:251 → 329

TPR_12; Tetratricopeptide repeat
NM_001081959.1 → NP_001075428.1 kinesin light chain 1 isoform 1H

See identical proteins and their annotated locations for NP_001075428.1

Status: REVIEWED

Description

Transcript Variant: This variant (h) uses an alternate in-frame splice site in the 3' coding region, compared to variant d. The resulting protein (isoform 1H) is shorter than isoform 1D.

Source sequence(s)

AK031309, AU035615, BC055744

Consensus CDS

CCDS49183.1

UniProtKB/TrEMBL

Q7M700, Q7TNF4

Related

ENSMUSP00000113997.2, ENSMUST00000122300.8

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13374
Location:296 → 337

TPR_10; Tetratricopeptide repeat

pfam13424
Location:251 → 329

TPR_12; Tetratricopeptide repeat
NM_001361605.1 → NP_001348534.1 kinesin light chain 1 isoform 1I

Status: REVIEWED

Source sequence(s)

AK031309, AU035615, BC055744, CN456461, CX734724

UniProtKB/TrEMBL

Q7M6Z9

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat

cl25732
Location:35 → 149

SMC_N; RecF/RecN/SMC N terminal domain
NM_001361609.1 → NP_001348538.1 kinesin light chain 1 isoform 1J

Status: REVIEWED

Source sequence(s)

AC152065, AK031309, AY753300, BC055744, BY783968

UniProtKB/TrEMBL

Q7M6Z7

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat

cl25732
Location:35 → 149

SMC_N; RecF/RecN/SMC N terminal domain
NM_001361610.1 → NP_001348539.1 kinesin light chain 1 isoform 1K

Status: REVIEWED

Source sequence(s)

AC152065, AK031309, BC055744, BY783968

UniProtKB/TrEMBL

Q7M6Z7

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat

cl25732
Location:35 → 149

SMC_N; RecF/RecN/SMC N terminal domain
NM_001361611.1 → NP_001348540.1 kinesin light chain 1 isoform 1L

Status: REVIEWED

Source sequence(s)

AC152065, BE946775

UniProtKB/TrEMBL

Q7M701

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat

cl25732
Location:35 → 149

SMC_N; RecF/RecN/SMC N terminal domain
NM_001361612.1 → NP_001348541.1 kinesin light chain 1 isoform 1M

Status: REVIEWED

Source sequence(s)

AC152065, BE946775

UniProtKB/TrEMBL

Q7M702

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat

cl25732
Location:35 → 149

SMC_N; RecF/RecN/SMC N terminal domain
NM_001361613.1 → NP_001348542.1 kinesin light chain 1 isoform 1N

Status: REVIEWED

Source sequence(s)

AC152065, BC055744, BE946775, BY783968, CD775356, CJ249676, CX734724

UniProtKB/TrEMBL

Q7M6Z8

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat

cl25732
Location:35 → 149

SMC_N; RecF/RecN/SMC N terminal domain
NM_001361614.1 → NP_001348543.1 kinesin light chain 1 isoform 1O

Status: REVIEWED

Source sequence(s)

AC152065, BC055744, BE946775, BY783968, CJ249676, CX734724

UniProtKB/Swiss-Prot

O88447

UniProtKB/TrEMBL

Q7M703

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat

cl25732
Location:35 → 149

SMC_N; RecF/RecN/SMC N terminal domain
NM_001422149.1 → NP_001409078.1 kinesin light chain 1 isoform 1P

Status: REVIEWED

Source sequence(s)

AC152065
NM_008450.2 → NP_032476.2 kinesin light chain 1 isoform 1A

See identical proteins and their annotated locations for NP_032476.2

Status: REVIEWED

Description

Transcript Variant: This variant (a) uses an alternate splice site and alternate exon in the 3' coding region and 3' UTR, compared to variant d. The resulting protein (isoform 1A) is shorter and has a distinct C-terminus, compared to isoform 1D.

Source sequence(s)

AU035615, BC055744, BE946775, CJ249676, CX734724

Consensus CDS

CCDS49182.1

UniProtKB/Swiss-Prot

O88447

UniProtKB/TrEMBL

Q5UE59, Q7M703

Related

ENSMUSP00000113171.2, ENSMUST00000118471.8

Conserved Domains (3) summary

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13374
Location:296 → 337

TPR_10; Tetratricopeptide repeat

pfam13424
Location:251 → 329

TPR_12; Tetratricopeptide repeat

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000078.7 Reference GRCm39 C57BL/6J

Range

111724869..111774278

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036157198.1 → XP_036013091.1 kinesin light chain 1 isoform X2

UniProtKB/TrEMBL

Q7M700
XM_030246566.2 → XP_030102426.1 kinesin light chain 1 isoform X6

UniProtKB/TrEMBL

Q7M6Z7

Conserved Domains (3) summary

TIGR02168
Location:35 → 149

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat
XM_036157197.1 → XP_036013090.1 kinesin light chain 1 isoform X1

UniProtKB/TrEMBL

A0A5F8MPZ2, Q7M700

Related

ENSMUSP00000159193.2, ENSMUST00000239017.2

Conserved Domains (3) summary

TIGR02168
Location:35 → 149

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat
XM_036157199.1 → XP_036013092.1 kinesin light chain 1 isoform X3

UniProtKB/TrEMBL

Q7M6Z9

Conserved Domains (3) summary

TIGR02168
Location:35 → 149

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

sd00006
Location:297 → 325

TPR; TPR repeat [structural motif]

pfam13424
Location:296 → 370

TPR_12; Tetratricopeptide repeat
XM_030246565.2 → XP_030102425.1 kinesin light chain 1 isoform X4

Conserved Domains (3) summary

TIGR02168
Location:35 → 149

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

sd00006
Location:344 → 372

TPR; TPR repeat [structural motif]

pfam13424
Location:342 → 417

TPR_12; Tetratricopeptide repeat