GEO DataSets

(Submitter supplied) Gene expression profiles of 39 human sarcoma samples (GSM 52571-GSM52609) and 15 control samples (GSM52556-GSM52570) were assessed using Affymetrix HG U133A oligonucleotide arrays. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1209

Platform:

GPL96

54 Samples

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Expression profiling of soft tissue sarcoma samples. Hypoxic regions often develop in tumors as they increase in size. Results provide insight into the expression of hypoxia-related genes in sarcomas.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 23 tissue sets

Platform:

GPL96

Series:

GSE2719

54 Samples

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(Submitter supplied) Tumor hypoxia is not a stable phenomenon but cycles between periods of deep hypoxia and reoxygenation. Cyclic hypoxia originates from heterogeneities in red blood cell flux and from the permanent remodelling of the angiogenic vascular network. Endothelial cells lining tumor blood vessels are therefore also influenced by cyclic hypoxia. The gene expression pattern promoted by cyclic hypoxia differs from those observed under normoxia and even continuous hypoxia. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

6 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Cell lines. HEK293 human embryonic kidney cells were obtained from ATCC (American Type Culture Collection, Manassas, VA) and were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Heat inactivated, Hyclone, Logan, Utah) and 1X antibiotics/glutamine (100 units/ml penicillin, 100ug/ml streptomycin, 292ug/ml L-Glutamine sulfate, all from Invitrogen Corp, Carlsbad, CA.), under either hypoxic (1% O2) or normoxic (21% O2) conditions at 37C in a tissue culture incubator. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2018

Platform:

GPL1528

10 Samples

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Analysis of HEK293T embryonic kidney cells subjected to hypoxia or overexpressing hypoxia-inducible factor (HIF)-1, degradation resistant HIF-1, or HIF-2. Results provide insight into the respective roles of HIF-1 and HIF-2 in the cellular response to hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 4 protocol sets

Platform:

GPL1528

Series:

GSE2020

10 Samples

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(Submitter supplied) Two pancreatic cancer cell lines with different metastatic and growth potential were compared under hypoxic conditions and under normal atmospheric oxygen pressure. The FG cell lines shows very few metastases and slow growth in mouse xenograft models. L3.6pl, derived from FG by cycles re-implantation of metastatic cells obtained after orthotopic tumor growth in nude mice, shows high motility, aggressive growth and very high metastatic potential By comparison of the two cell lines under different oxygen concentration we tried to simulate in vivo conditions of tumors at different growth stages. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) Overall study: Identification of PDGF-dependent patterns of gene expression in U87 glioblastoma cells. RNA was obtained from triplicate dishes of 5 different groups of U87 cells, each (total 15) analyzed with one U95 microarray chip. Three different comparisons were made: 1) Clone 3.1 (34580-34582) vs. clone 3.3 (34583-34585) vs. parent U87 (34592-34594). Purpose: demonstrate that the gene expression profiles between these 3 cell lines are not different, so they could be pooled as a single untreated group. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1730

Platform:

GPL8300

15 Samples

Download data: CEL, EXP

Analysis of U87 glioblastoma (GBM) cell clones overexpressing a dominant-negative form of platelet-derived growth factor (PDGF) A subunit to inactive the PDGF autocrine signaling loop. The autocrine PDGF loop is a hallmark of GBM. Results identify genes regulated by oncogenic PDGF signaling.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 genotype/variation sets

Platform:

GPL8300

Series:

GSE1923

15 Samples

Download data: CEL, EXP

(Submitter supplied) Whole genome array expression analysis was performed to identify changes in gene expression associated with the downregulation of the Core Binding Factor subunit CBFbeta.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

6 Samples

Download data: TXT

(Submitter supplied) Normal, premalignant and various histological subtypes of testicular germ cell tumor (TGCT) tissues were hybridized against Universal Human Reference RNA (Stratagene) onto Agilent 60mer oligo microarrays (GEO accession no GPL885). In vitro time series of two TGCT cell lines, NTERA2 and 2102Ep, treated with retinoic acid for 0, 3, and 7 days were also included. The data set (30 hybridizations) is particularly useful for comparisons between various histological subtypes of TGCT versus each other or versus normal testis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL885

30 Samples

Download data: TIFF

(Submitter supplied) Summary: Astrocytomas can be categorized as either low grade or high grade (glioblastoma). Low grade astrocytomas are not generally aggressive tumors whereas glioblastomas are and in turn have a high mortality rate. The purpose of this experiment is to identify genetic differences between the two types. Hypothesis: There will be a difference in RNA expression between high grade and low grade tumors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2853

Platforms:

GPL96 GPL8300

20 Samples

Download data: CEL

Comparison of low and high grade astrocytoma brain tumors. Results provide insight into the molecular differences between the two types of tumors.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 disease state, 2 other sets

Platform:

GPL8300

Series:

GSE3185

16 Samples

Download data: CEL

(Submitter supplied) Cultures of human aortic (HAEC) and pulmonary artery endothelial cells (HPAEC) were exposured to short-term chronic hypoxia (1% O2) for either 0h, 8h or 24h Keywords: Time course, cell-type comparison

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

6 Samples

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(Submitter supplied) We recently described that tumor-derived exosomes containing the tetraspanin Tspan8 induce angiogenesis. However, not much is known about exosome-endothelial cell interactions and there are no clues on the role of tetraspanins, which are a constitutive component of exosomes. We used the stably Tspan8 expressing rat adenocarcinoma cell line AS-Tspan8, to explore how exosomal Tspan8 supports angiogenesis. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6101

12 Samples

Download data: TXT

(Submitter supplied) The primary aim of this study was to evaluate the changes in hepatocyte gene expression under short-term hypoxic conditions in wild type and HIF-1a null cultures. To this end, hypoxia treated cultures were subjected to incubation with 1% O2/5% CO2/94% N2 at 37° C for eight hours prior to RNA isolation. Duplicate normoxic controls were established from separate animals wherein cultures were untreated and treated with Adbgal. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1648

Platform:

GPL81

10 Samples

Download data: CEL

Analysis of hypoxia-inducible factor-1alpha (HIF-1alpha) null mutant hepatocytes subjected to hypoxic conditions for 8 hours. The HIF-1alpha transcription factor is a key regulator of oxygen homeostasis. Results provide insight into hepatocyte gene expression changes induced by short-term hypoxia.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 stress sets

Platform:

GPL81

Series:

GSE1321

10 Samples

Download data: CEL

(Submitter supplied) The aim of the experiment was to analyse the gene expression differences between MEFs containing TAp73 or without TAp73, at basal levels in normal culture conditions, and upon treatment with hypoxia (by incubation in 1% oxygen) for 4 or 8 hrs. The analysis indicate that absence of TAp73 leads to changes in multiple cellular and biological processes.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

12 Samples

Download data: IDAT, TXT

(Submitter supplied) VEGF165 is one of the key regulators of tumor development. Using HCT116 cells transfected with full-length vegf mRNA, full-length vegf mRNA with mutations of H9D and L14E, mutated vegf mRNAs lacking untranslated regions (UTRs), and 5’UTR mutated between nt 591 and nt 746, we found that vegf 5’UTR resided an anti-apoptotic activity against chemotherapy independently of VEGF165. We next established HCT116 clones stably expressing vegf 5’UTR or the mutated vegf 5’UTR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL885

1 Sample

Download data: TXT

(Submitter supplied) Iron-deficiency affects 500 million people, yet the molecular role of iron in gene expression remains poorly characterized. Moreover, the alterations in global gene expression after iron chelation remains unclear and are important to assess for understanding the molecular pathology of iron-deficiency and the biological effects of iron chelators. We assessed the effect on whole genome gene expression of two iron chelators (desferrioxamine and 2-hydroxy-1-napthylaldehyde isonicotinoyl hydrazone) that have markedly different permeability properties. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

3 Samples

Download data: CEL, CHP

(Submitter supplied) PHD4 regulates the expression of Hypxia-inducible Factor 2 (HIF-2) alpha in LM8 osteosarcoma cells. PHD4 overexpression inhibits the growth of experimental tumor in syngenic mice but stimulates angiogenesis via Transforming Growth-Factor (TGF)-alpha. We used microarrays to investigate the gene expression of stable LM8 cell line overexpressing human PHD4 in comparison to the LM8 control line, transfected with the empty vector.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

2 Samples

Download data: CEL