GEO DataSets

Analysis of nasal epithelia exposed to various doses of the carcinogenic pollutant formaldehyde for up to 19 days. Results used as a case study to validate a method that integrates benchmark dose calculations with gene ontology analysis to examine genomic dose-response data.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 2 agent, 7 dose, 2 protocol, 5 time sets

Platform:

GPL1355

Series:

GSE7002

98 Samples

Download data: CEL

(Submitter supplied) Formaldehyde, an important industrial chemical, is used for multiple commercial purposes throughout the industrialized world. This simple, one carbon aldehyde is a natural metabolite formed in cells throughput the body. However, it is also a rodent nasal carcinogen, when inhaled by rats every day for two-years at irritant concentrations. High tumor incidences occur at concentration of 10 ppm and above; no tumors are observed at concentrations below 6.0 ppm. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2680

Platform:

GPL1355

98 Samples

Download data: CEL

(Submitter supplied) Formaldehyde (FA), an endogenous cellular aldehyde, is a rat nasal carcinogen. In this study, concentration- and exposure-duration transitions in FA mode of action (MOA) were examined with pharmacokinetic (PK) modeling for tissue formaldehyde acetal (FAcetal) and glutathione (GSH) and with histopathology and gene expression studies for tissue responses in nasal epithelium from rats exposed to 0, 0.7, 2, 6, 10 or 15 ppm FA 6 hr/day for 1, 4 or 13 weeks. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

72 Samples

Download data: CEL

(Submitter supplied) Using various exposure conditions, we studied the induction of DNA-protein crosslinks (DPX) by formaldehyde (FA) and their removal in primary human nasal epithelial cells (HNEC). DPX were indirectly measured by the alkaline comet assay as the reduction of gamma ray – induced DNA migration. DPX are the most relevant primary DNA alterations induced by FA and the comet assay is a very sensitive method for the detection of FA-induced DPX. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

39 Samples

Download data: CEL

(Submitter supplied) Male Sprague-dawley rats were exposed to saline, isopropyl alcohol, 1mg/kg, 3mg/kg or 6 mg/kg sulfur mustard for 30 min, 1 hr, 3 hr, 6 hr, or 24 hr before analysis of lung tissue by oligonucleotide array analysis. Keywords = sulfur mustard Keywords = rat Keywords = lung Keywords = bis-(2-chloroethyl) sulfide Keywords: ordered

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS1027

Platform:

GPL341

154 Samples

Download data: CEL, EXP, RPT

Analysis of lungs from Sprague Dawley males following injection with 1, 3, or 6 mg/kg of sulfur mustard (SM) bis-(2-chloroethyl) sulfide, a carcinogenic alkylating agent. Lungs examined at various time points up to 24 hours postinjection. Results provide insight into SM-induced lung injury.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 4 dose, 2 protocol, 5 time sets

Platform:

GPL341

Series:

GSE1888

154 Samples

Download data: CEL, EXP, RPT

(Submitter supplied) Two-year rodent bioassays play a central role in evaluating both the carcinogenic potential of a chemical and generating quantitative information on the dose-response behavior for chemical risk assessments. The bioassays involved are expensive and time-consuming, requiring nearly lifetime exposures (two years) in mice and rats and costing $2 to $4 million per chemical. Since there are approximately 80,000 chemicals registered for commercial use in the United States and 2,000 more are added each year, applying animal bioassays to all chemicals of concern is clearly impossible. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

242 Samples

Download data: CEL, CHP

(Submitter supplied) Concentration- and time-dependent genomic changes in the mouse urinary bladder following exposure to arsenate in drinking water for up to twelve weeks. Inorganic arsenic (Asi) is a known human bladder carcinogen. The objective of this study was to examine the concentration dependence of the genomic response to Asi in the urinary bladders of mice. C57BL/6J mice were exposed for 1 or 12 weeks to arsenate in drinking water at concentrations of 0.5, 2, 10, and 50 mg As/L. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

49 Samples

Download data: CEL

(Submitter supplied) The alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) induces cellular DNA damages and other comprehensive alterations that lead to chromosomal aberrations, mutations, tumor initiations, and cell death. However, the molecular mechanism of MNNG-induced cellular stress remains unclear.We have genome-wide analyzed early transcriptional responses of human FL amnion epithelial cells after exposure to three relatively low doses of MNNG (0.2, 1.0, and 10.0µM),and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL97

16 Samples

Download data: CEL, EXP

(Submitter supplied) d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3643

Platform:

GPL1355

24 Samples

Download data: CEL

Analysis of forebrains of animals treated with up to 500 mg/kg D-serine for 96 hours. D-serine is involved in many physiological processes through its interaction with the glycine binding site of the NMDA receptor. Results provide insight into the impact of D-serine exposure on neuronal functions.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 6 dose sets

Platform:

GPL1355

Series:

GSE10748

24 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rattus norvegicus

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL14854

57 Samples

Download data: TXT

(Submitter supplied) In addition to gaining knowledge on in vivo miRNA responses to formaldehyde, we set out to relate these miRNA responses to transcriptional profiles modified by formaldehyde. Rats were exposed by inhalation to either 0 or 2 ppm formaldehyde (6 hours/day) for 28 days. Genome-wide transcriptional profiles and associated signaling pathways were assessed within the nasal respiratory mucosa and circulating mononuclear white blood cells (WBC).

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL6247

24 Samples

Download data: CEL

(Submitter supplied) MicroRNAs (miRNAs) are critical regulators of gene expression, yet much remains unknown regarding miRNA changes resulting from environmental exposures and whether they influence pathway signaling across various tissues and time. To gain knowledge on these novel topics, we set out to investigate in vivo miRNA responses to inhaled formaldehyde, an important air pollutant known to disrupt miRNA expression profiles. more...

Organism:

Rattus norvegicus

Type:

Non-coding RNA profiling by array

Platform:

GPL14854

57 Samples

Download data: TXT