GEO DataSets

(Submitter supplied) The underlying pathogenic mechanisms of prion infection are not well characterized. To study the effect of prion infection on gene expression in neuronal cell cultures, a neuroblastoma (N2a) cell clone was infected with either the mouse adapted prion strain 22L or exposed to uninfected brain homogenate as a negative control. Large scale expression analysis was performed using a cDNA microarray chip comprising about 21,000 spotted ESTs. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3697

8 Samples

Download data: GPR

(Submitter supplied) Prion infection results in progressive neurodegeneration of the central nervous system invariably resulting in death. The pathological effects of prion diseases in the brain are morphologically well defined, such as gliosis, vacuolation, and the accumulation of disease-specific protease-resistant prion protein (PrPSc). However, the underlying molecular events that lead to the death of neurons are poorly characterised. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6412 GPL4134

39 Samples

Download data: TXT

(Submitter supplied) While prion infections have been extensively characterized in the laboratory mouse, little is known regarding the molecular responses to prions in other rodents. To explore these responses and make comparisons, we generated a prion disease in the laboratory rat by successive passage of mouse RML prions. Here we describe the accumulation of prions and associated pathology in the rat and describe the transcriptional impact throughout prion disease. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

24 Samples

Download data: CEL

(Submitter supplied) We found the genome-wide co-binding of QKI-5 and SREBP2. Notably, the genomic distribution analysis revealed that the co-binding events between QKI-5 and SREBP2 highly occurred at the regions of the promoter/transcription start site (TSS), where active transcription was taken place in a lens cell-specific manner, which was indicated by POL II binding events. Cellular pathway analysis of the genes whose promoters were co-bound by QKI-5, SREBP2, and POL II showed a significant enrichment of the SREBP2-medaited cholesterol biosynthesis pathway, and QKI depletion led to reduced co-occupancies of SREBP2 and POL II on the promoters of the genes involved in cholesterol biosynthesis. more...

Organism:

Homo sapiens; Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

4 related Platforms

17 Samples

Download data: BED, NARROWPEAK

(Submitter supplied) Prion diseases typically have long pre-clinical incubation periods during which time the infectious prion particle and infectivity steadily propagate in the brain. Abnormal neuritic sprouting and synaptic deficits are apparent during pre-clinical disease, however, gross neuronal loss is not detected until the onset of the clinical phase. The molecular events that accompany early neuronal damage and ultimately conclude with neuronal death remain obscure. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

38 Samples

Download data: TXT

(Submitter supplied) In mammals, O2 and CO2 levels are tightly regulated and are altered under various pathological conditions. While the molecular mechanisms that participate in O2 sensing are well characterized, little is known regarding the signaling pathways that participate in CO2 signaling and adaptation. Here, we show that CO2 levels control a distinct cellular transcriptional response that differs from mere pH changes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21626

20 Samples

Download data: XLSX

(Submitter supplied) Prion diseases are fatal transmissible neurodegenerative conditions of humans and animals that arise through neurotoxicity induced by PrP misfolding. The cellular and molecular mechanisms of prion-induced neurotoxicity remain undefined. Understanding these processes will underpin therapeutic and control strategies for human and animal prion diseases, respectively. Prion diseases are difficult to study in their natural hosts and require the use of tractable animal models. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL13304 GPL21306

36 Samples

Download data: TXT, XLSX

(Submitter supplied) Flavonoids are polyphenolic compounds with potent anti-oxidant and free radical scavenging activities. Here, we examined the cytoprotective actions of Quercetin-3-glucoside (Q3G) against oxidative stress induced by hydrogen peroxide. Pre-treatment of the neuroblastoma cells, SH-SY5Y with Q3G for 18 h reduced cell death after a brief exposure to hydrogen peroxide. The cytoprotective effects of Q3G were associated with decreased free radical generation suggesting that this mechanism accounts for Q3G-mediated cytoprotection. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL3963 GPL3964

8 Samples

Download data

(Submitter supplied) Flavonoids are polyphenolic compounds with potent anti-oxidant and free radical scavenging activities. Here, we examined the cytoprotective actions of Quercetin-3-glucoside (Q3G) against oxidative stress induced by hydrogen peroxide. Pre-treatment of the neuroblastoma cells, SH-SY5Y with Q3G for 18 h reduced cell death after a brief exposure to hydrogen peroxide. The cytoprotective effects of Q3G were associated with decreased free radical generation suggesting that this mechanism accounts for Q3G-mediated cytoprotection. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL3963 GPL3964

8 Samples

Download data

(Submitter supplied) A comparison of prion infected and non-infected samples from neuroblastoma cells (N2a), and a comparison of prion infected and non-infected samples from hypothalmus cells (GT1). 11 dual-color DNA-chip hybridizations of cDNAs were made. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1413

11 Samples

Download data

(Submitter supplied) Study on gene expression in multifunctional protein 2 deficient mice. Liver samples of two days old mice in normal conditions are used. In total 8 arrays were hybridized corresponding to 4 KO mice and 4 WT mice Results: Cholesterol synthesis is induced and ppar alpha targets also differentially expressed between KO and WT. Keywords: Knockout gene expression study; genetic modification

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3468

Platform:

GPL1261

8 Samples

Download data: CEL

Analysis of livers of 2 day old mutants lacking multifunctional protein 2 (Mfp2), an enzyme of the peroxisomal beta-oxidation pathway. Mfp2 deficiency results in extensive metabolic and pathological abnormalities starting in the postnatal period and leads to death by the age of 6 months.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE10895

8 Samples

Download data: CEL

(Submitter supplied) To reveal the molecular mechanism underling necrotic neuronal cell death caused by norephedrine, we examined alteration of gene expression profile during norephedrine exposure in human neuroblastoma SH-SY5Y cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

3 Samples

Download data: TXT

(Submitter supplied) A key event in the pathogenic process of prion diseases is the conversion of the cellular prion protein (PrPC) to an abnormal and protease-resistant isoform (PrPSc). Mice lacking PrP are resistant to prion infection, and down-regulation of PrPC during prion infection prevents neuronal loss and the progression to clinical disease. These results are suggestive of the potential beneficial effect of silencing PrPC during prion diseases. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4352

Platform:

GPL8321

14 Samples

Download data: CEL

Analysis of hippocampi from cellular prion protein (PrPC)-deficient, FVB newborns (4.5-day-old) and adults (3-mo-old). PrPc protein is widely expressed throughout the CNS; mice lacking PrP are resistant to prion infection. Results provide insight into the influence of PrPC on the developing CNS.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 development stage, 2 genotype/variation sets

Platform:

GPL8321

Series:

GSE21718

14 Samples

Download data: CEL

(Submitter supplied) The brain is the most cholesterol-rich organ in the body, most of which comes from in situ synthesis. Here we demonstrate that in insulin-deficient diabetic mice, there is a reduction in expression of the major transcriptional regulator of cholesterol metabolism, SREBP-2, and its downstream genes in the hypothalamus and other areas of the brain, leading to a reduction in brain cholesterol synthesis and synaptosomal cholesterol content. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5344

Platform:

GPL8321

17 Samples

Download data: CEL

Analysis of hypothalamus from streptozotocin-induced diabetic males (a model of insulin-deficient, type 1 diabetes). The hypothalamus controls the endocrine system, appetite and energy balance. Results provide molecular insight into how diabetes affects hypothalamic function.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 disease state sets

Platform:

GPL8321

Series:

GSE62013

17 Samples

Download data: CEL

(Submitter supplied) The Golgi apparatus plays a central role in the protein glycosylation where it is required for secretory trafficking. Abnormal morphology of the Golgi apparatus has been widely observed in neurodegenerative disease. Golgi pH regulator (GPHR) is an anion channel essential for acidification of the Golgi lumen and its essential for normal morphology and function of the Golgi apparatus. To address the Golgi dysfunction in neuronal cells, we established brain-specific GPHR knockout mice (GPHRf/f;Nes).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL21163

2 Samples

Download data: TXT

(Submitter supplied) Selective vulnerability is an enigmatic feature of neurodegenerative diseases (NDs), whereby a widely expressed protein causes lesions in specific cell types and brain regions. Using the RiboTag method in mice, translational responses of five neural subtypes to acquired prion disease (PrD) were measured. Pre-onset and disease onset timepoints were chosen based on longitudinal electroencephalography (EEG) that revealed a gradual increase in theta power between 10- and 18-weeks after prion injection, resembling a clinical feature of human PrD. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

98 Samples

Download data: CSV