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Series GSE4179 Query DataSets for GSE4179
Status Public on Feb 09, 2006
Title A function for interleukin 2 in Foxp3-expressing regulatory T cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Regulatory T cells (Treg cells) expressing the forkhead family transcription factor Foxp3 are critical mediators of dominant immune tolerance to self. Most Treg cells constitutively express the high-affinity interleukin 2 (IL-2) receptor alpha-chain (CD25); however, the precise function of IL-2 in Treg cell biology has remained controversial. To directly assess the effect of IL-2 signaling on Treg cell development and function, we analyzed mice containing the Foxp3gfp knock-in allele that were genetically deficient in either IL-2 (Il2-/-) or CD25 (Il2ra-/-). We found that IL-2 signaling was dispensable for the induction of Foxp3 expression in thymocytes from these mice, which indicated that IL-2 signaling does not have a nonredundant function in the development of Treg cells. Unexpectedly, Il2-/- and Il2ra-/- Treg cells were fully able to suppress T cell proliferation in vitro. In contrast, Foxp3 was not expressed in thymocytes or peripheral T cells from Il2rg-/- mice. Gene expression analysis showed that IL-2 signaling was required for maintenance of the expression of genes involved in the regulation of cell growth and metabolism. Thus, IL-2 signaling seems to be critically required for maintaining the homeostasis and competitive fitness of Treg cells in vivo.
Keywords: cell type comparison
 
Overall design We isolated Foxp3+ CD4+ T cells from Il2-/- mice treated with PBS or recombinant IL-2 for gene expression analysis using whole-genome oligonucleotide microarrays. To identify gene expression changes resulting from IL-2 deficiency and subsequent IL-2 stimulation in Treg cells, we directly compared expression profiles with each other and with expression profiles of Foxp3+ CD4+ T cells isolated from wild-type mice.
 
Contributor(s) Fontenot JD, Rasmussen JP, Gavin MA, Rudensky AY
Citation(s) 16227984
Submission date Feb 03, 2006
Last update date Feb 11, 2019
Contact name Jeffrey Rasmussen
E-mail(s) rasmuss@u.washington.edu
Phone 2065439058
Organization name University of Washington
Department Immunology
Lab Rudensky
Street address
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (5)
GSM44980 CD4+ CD25hi Foxp3+ T cells
GSM44981 CD4+ CD25lo Foxp3+ T cells
GSM44982 CD4+ CD25+ Foxp3- T cells
Relations
BioProject PRJNA94983

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4179_RAW.tar 17.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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