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Series GSE4179 Query DataSets for GSE4179
Status Public on Feb 09, 2006
Title A function for interleukin 2 in Foxp3-expressing regulatory T cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Regulatory T cells (Treg cells) expressing the forkhead family transcription factor Foxp3 are critical mediators of dominant immune tolerance to self. Most Treg cells constitutively express the high-affinity interleukin 2 (IL-2) receptor alpha-chain (CD25); however, the precise function of IL-2 in Treg cell biology has remained controversial. To directly assess the effect of IL-2 signaling on Treg cell development and function, we analyzed mice containing the Foxp3gfp knock-in allele that were genetically deficient in either IL-2 (Il2-/-) or CD25 (Il2ra-/-). We found that IL-2 signaling was dispensable for the induction of Foxp3 expression in thymocytes from these mice, which indicated that IL-2 signaling does not have a nonredundant function in the development of Treg cells. Unexpectedly, Il2-/- and Il2ra-/- Treg cells were fully able to suppress T cell proliferation in vitro. In contrast, Foxp3 was not expressed in thymocytes or peripheral T cells from Il2rg-/- mice. Gene expression analysis showed that IL-2 signaling was required for maintenance of the expression of genes involved in the regulation of cell growth and metabolism. Thus, IL-2 signaling seems to be critically required for maintaining the homeostasis and competitive fitness of Treg cells in vivo.
Keywords: cell type comparison
Overall design We isolated Foxp3+ CD4+ T cells from Il2-/- mice treated with PBS or recombinant IL-2 for gene expression analysis using whole-genome oligonucleotide microarrays. To identify gene expression changes resulting from IL-2 deficiency and subsequent IL-2 stimulation in Treg cells, we directly compared expression profiles with each other and with expression profiles of Foxp3+ CD4+ T cells isolated from wild-type mice.
Contributor(s) Fontenot JD, Rasmussen JP, Gavin MA, Rudensky AY
Citation(s) 16227984
Submission date Feb 03, 2006
Last update date Feb 11, 2019
Contact name Jeffrey Rasmussen
Phone 2065439058
Organization name University of Washington
Department Immunology
Lab Rudensky
Street address
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (5)
GSM44980 CD4+ CD25hi Foxp3+ T cells
GSM44981 CD4+ CD25lo Foxp3+ T cells
GSM44982 CD4+ CD25+ Foxp3- T cells
BioProject PRJNA94983

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GSE4179_RAW.tar 17.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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